Description
Holomonitor 600 M3 Tracking
System
Measurement technique: single wavelength transmission digital holographic microscopy
Image types: intensity and quantitative phase contrast image (DH mode)
phase contrast image (PC mode)
Light source: monochromatic laser source (DH mode)
Luxeon 1 watt LED (PC mode)
Sample stage: manual or automated XY stage up to 150 mm × 120 mm travel
range automated Z stage up to 20mm travel range
Camera 1280 × 1024 pixels, 8 or 10 bits (hologram)
2048 × 1536 pixels, 8 bit RGB (phase contrast)
Available objectives: standard objectives 10-100x
Objective mounting: 4-position objective turret with manual or automated function
Computer: PC workstation with latest Intel processor.
Software: Holostudio™ imaging and cell analyze software
Performance
Lateral resolution: objective dependent, down to 500 nm 100x objective
Field of view: objective dependent, up to 4 mm
Working distance: objective dependent, from 0.3 to 18 mm
Digital focusing range: up to 50× depth of field (objective dependent)
Grabbing time: down to 4 μs in a single image grab (no scanning mechanism,
insensitive to vibrations)
Spatial sampling: 1024 × 1024 pixels (hologram)
Acquisition rate: 27.5 fps (1024 × 1024 pixels)
Reconstruction rate: 4 fps (512 × 512 pixels), 1 fps (1024 × 1024 pixels)
Sample illumination: ~0.1 mW/cm2
Maximum container height: 60mm
Power requirements
Input voltage: 230 VAC - 50/60 Hz
Power requirements
(w/o computer):
max. 83 W
Dimensions & weight
Dimensions (L × W × H): 650 × 360 × 560 mm
Weight: 35 kg
Samples
Cells Eukaryotic adherent monolayer cells in any standard cell culture
vessel or suspension cells in IBIDI µ-slides.
Culture vessels Any standard culture flask or petri dish, multi well plates down to
24-wells, any IBIDI µ-slide
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